Bioactivity behavior and cytotoxicity of SiO2-NaO2-CaO-P2O3-RE2O3(RE=Yb, Gd) glasses

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Abstract
02-097 Giulia Piagentini Delpino Zambanini, T.(Federal University of Abc); Piagentini Delpino, G.(Federal University of Abc); Marchi, J.(Universidade de São Paulo); Although rare earth elements (RE) can be used in different biological applications, bioactiveglasses containing rare earth elements (BG-RE) have been poorly explored as a biomaterial that could ally osteoconduction with other propertieslike brachytherapy, diagnostic imaging, among others. In this work, we evaluated the bioactivity behavior and cytotoxicity properties of melt-derived BG-RE based on the 48SiO2-27NaO2-19CaO-6P2O3-2,5RE2O3 (RE=Yb, Gd) system. Samples were submitted to dissolution tests in Tris-HCl and bioactivity tests in Simulated Body Fluid (SBF) solution up to 7 days, and cytotoxicity was evaluated using dental pulp stem cells. In the dissolution test, glass dissolution kinetics was determined by measuring the ionic concentration of the dissolved glasses by induced coupled plasma optical emission spectrometry. In the samples immersed in the SBF solution,the formation of hydroxyapatite on glass surface was analyzed through X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy and Energy Dispersive Spectroscopy. For cytotoxicity assay, cellswere incubated in a conditioned medium containing ionic dissolution products of the glasses for 72h, and afterwards the cell viability was tested using MTT essay. Comparison between BG and BG-REresults showed that the presence of Gd and Yb in the glass structuredecreased the glass dissolution kinetics, suggesting that Si-O-RE bonds may be stronger than other bonds present in the glass. However, this change in dissolution kinetics led to not very significant changes in terms of bioactivity and cell viability, suggesting that BG-RE are promise materials for new biological applications, and should be more explored in the field of biomaterials.
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