Biodegradable Polymers grafted into Multifunctional Mesoporous Silica Nanoparticles for Gene Delivery

Reference Presenter Authors
02-022 EgÍdio Paulo Francisco Nhavene Nhavene, E.P.(Centro de Desenvolvimento da Tecnologia Nuclear); Sousa, E.M.(Centro de Desenvolvimento da Tecnologia Nuclear); Biodegradable polymer possesses significant potential of applications in different fields in nanobiotechnology since flexibility gives rise to materials with great physical and mechanical property diversity. The Poly-caprolactone (PCL) has ability to form scaffolds which adhere into surface of mesoporous silica nanoparticles (MSNs) through porous networks created. The novel characteristics of the developed PCL/MSNs such as very low in vivo degradation rate, high drug permeability, the ordered pore network, uniform and tunable size and shape of the particles, high pore volume and surface area, non-toxicity and biocompatibility among others are responsible for its favorable gene delivery device and makes this conjugation a very good biomaterial for this application. In the present study, we investigated the synthesis of silica nanoparticles and PCL covalently attached on its surface and its use as a potential pTREX-GPF plasmid. The physical-chemical and morphological characterizations, as well as the applicability of functionalized MSNs as platforms for gene delivery were assessed. Our results confirmed that MSNs were successfully functionalized with PCL and kept their typical morphology and pore structure. Furthermore, their surface modification was successful. In vitro test of biocompatibility and cytotoxicity suggests the ability of MSNs to support passive uptake and indicated the potential of this material as gene delivery systems into T.cruzi parasites.  

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