Study of hydroxyapatite containing propolis of Brazilian origin: characterization, antimicrobial activity and cytotoxic effect materials

Reference Presenter Authors
02-031 Eliana Cristina Da Silva Rigo Rigo, E.C.(Universidade de São Paulo, Campus Pirassununga); Scatolini, A.M.(Universidade de São Paulo, Campus Pirassununga); Pugine, S.M.(Universidade de São Paulo, Campus Pirassununga); Vercik, L.C.(Universidade de São Paulo, Campus Pirassununga); Vercik, A.(Universidade de São Paulo, Campus Pirassununga); Melo, M.P.(Universidade de São Paulo, Campus Pirassununga); The aim of this study was to produce hydroxyapatite (HA) containing different types of propolis of Brazilian origin and to evaluate the possible antimicrobial activity materials. The ethanolic extracts of red, green and brown propolis (EEP) were obtained in alcoholic solution 80%. Green and red EEP (8 mg / mL and 20 mg / mL) were incorporated into the 10% (m/v) via atomization (spray drying), obtaining HA-GP8, HA-GP20, HA-RP8 and HA -RP20. The materials were characterized regarding the content of phenolic compounds and total flavonoids. The HA powders incorporated with propolis were evaluated against S. aureus by plate colony counting , minimum inhibitory concentration (MIC) and minimum bactericidal (MBC). The cytotoxicity of the materials was determined by cell viability calculation, which was made by the neutral red incorporation method. The bactericidal effect for all materials was observed within the interval of 0.5 (HA-RP20 and HA-GP20) to 1 hour (HA-RP8 and HA-GP8). The materials showed lower inhibitory activity (MIC) for HA-GP20 and HA-GP8 (175, 4 and 182.0 µg/mL) and for HA-RP20 and HA-RP8 (51.7 and 66.8 µg/mL) compared to EEP. However, higher bactericidal activity (MBC) was observed for HA-GP20 and HA-GP8 (701.5 and 728.0 µg/mL) and for HA-RP20 and HA-RP8 (206.5 and 267.0 µg/mL) when compared to the EEP. The cytotoxicity assay showed values for IC50 (concentration that reflects 50% of cellular viability) of 387.1 and 84.8 µg/mL for HA-GP8 and HA-RP8 samples, respectively. Considering the results obtained in this work, it is suggested that HA incorporated with propolis (HA-GP8 and HA-RP8) can be used as a possible antimicrobial agent, inhibiting the growth of S. aureus, respecting the maximum values for IC50.
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