## Antitumor activity of silver tungstate (Ag2WO4) irradiated by different methods

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Abstract
02-047 Thaiane Alcarde Robeldo Robeldo, T.A.(Universidade Federal de São Carlos); Assis, M.(Universidade Federal de São Carlos); de Foggi, C.C.(Universidade Federal de São Carlos); Kubo, A.M.(Universidade Federal de São Carlos); Borra, R.C.(Universidade Federal de São Carlos); Longo, E.(INSTITUTO DE QUÍMICA - UNESP); Introduction. $\alpha$-Ag$2$WO$4$ is known for its use in several areas. The development of technologies that favors modifications of characteristics regarding its morphological structure, increase its capacity of use, including therapeutics. Modification may be associated with the formation of Ag nanoparticles on the surface during the irradiation processes. When $\alpha$-Ag$2$WO$4$ is irradiated with an electron beam, nanofilaments of Ag are added to the surface. In turn, when the material is irradiated with femtosecond laser, nanoparticles of spherical Ag are formed and added or not to the surface of the material, removing the equilibrium system. In this work we used $\alpha$-Ag$2$WO$4$ in the forms irradiated with electron and femtosecond laser with the objective of evaluating its antitumor power. Methods. $\alpha$-Ag$2$WO$4$ was synthesized by coprecipitation method. To electron irradiation, the sample was placed in a field emission gun scanning electron microscope (15kV for 5 minutes). To femtosecond irradiation, the sample was irradiated with a Ti:sapphire laser (30 fs; 800nm; 1kHz). A laser beam of 6 mm in diameter, at the 1/e2 point, and mean power of 200 mW was focused onto the surface of a pellet target of ?-Ag2WO4 with a 75 mm lens. In order to obtain the focused sample, an 8mm lens was used. MB49 (tumor) and BALB /3T3 (non-tumor) cell lines were used. Both strains were exposed to NPs at the following concentrations: 0.1; 0.25, 0.5 and 1.0 mM. After the 24-hour exposure, it performed the viability test, cytotoxicity and generation of reactive oxygen species. Results and Discussion. All of the forms of $\alpha$-Ag$2$WO$4$ used had effective abilities to cause death of tumor cells without causing toxicity to non-tumor cells. Here, we can assume that non-tumor cells could present some mechanism other than tumor cells that allow their survival when exposed to NPs.
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