CHARACTERIZATION AND EVALUATION OF THE BIOACTIVE POTENTIAL IN CEL2 BIOACTIVE GLASS-CERAMIC POROUS SCAFFOLDS LOADED WITH CHITOSAN MICROSPHERES

Reference Presenter Authors
(Institution)
Abstract
02-094 Esmeralda Villicaña Villicaña, E.(Instituto de Investigacion en Metalúrgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo); Aguilar, E.A.(Instituto de Investigacion en Metalúrgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo); Bioactive glasses offers the ability to adapt to soft and/or hard tissue. That the ability to regenerate human tissue through the production of hydroxyapatite depends on the porosity of the bioactive glass; the bioactive glass has a higher capacity, if this is more porous. It is advantageous to incorporate polymeric microspheres (MEs) into the scaffolds to delivery drugs. In this matter, chitosan is a good candidate because it is biocompatible, biodegradable and antimicrobial polymer. Glass-ceramic porous scaffold have been developed by the powder technology method, the starting glass CEL2 belongs to the system SiO2-P2O5-CaO-MgO-K2O-Na2O and has been synthesized by a conventional melting–quenching route. Chitosan MEs with spherical shape and a mean particle size 1 to 2.3 ?m loaded with acetaminophen were obtained by an oil-in-water single emulsion solvent evaporation method and they were incorporated on the surface of the glass-ceramic scaffolds by the immersion technique. This project aims to evaluate in vitro the biocompatibility and bioactivity of the scaffolds, whose reaction in SBF solution is the formation of hydroxyapatite. The characterization of MEs loaded-scaffolds before and after immersion in SBF was performed by scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. The MEs loaded-scaffolds showed more than 70% interconnected porosity and an average compressive strength of 4.5 ± .43 MPa. In vitro bioactivity assays were performed up to 21 days; scaffolds showed the formation of a hydroxyapatite layer since the first day of immersion in SBF, demonstrating their high bioactivity. Additionally NIH 3T3 mouse fibroblasts were seeded onto the scaffolds in vitro, further characterization, the CEL2 bioactive glass scaffolds with microspheres were biocompatible and showed good degree of cell proliferation and good viability. The results showed that the obtained devices are good candidates as scaffolds for bone-tissue engineering.
<< Back
Copyright © 2019 Metallum. All rights reserved.
Site produced by: SITESP.NET