Influence of manganese on the structure and in vitro behaviour of sol-gel derived bioactive glasses

Reference Presenter Authors
02-064 Breno Rocha Barrioni Barrioni, B.R.(Universidade Federal de Minas Gerais); Jones, J.R.(Imperial College); Naruphontjirakul, P.(Imperial College); Pereira, M.d.(Universidade Federal de Minas Gerais); Sol-gel synthesis of bioactive glasses (BGs) enables a versatile composition, allowing the incorporation of different ions with therapeutic properties in their structure. These ions can be released during the dissolution process of the glass, playing a specific role in the tissue regeneration. Among those ions, manganese has been shown to promote osteogenic differentiation and cell adhesion. Here, new sol-gel derived glasses derived from the 58S composition containing Mn were obtained. Infrared spectroscopy showed bands typical of bioactive glass for all samples and X-ray Diffraction patterns indicated that the typical amorphous structure was maintained after Mn incorporation. X-ray photoelectron spectroscopy indicated that manganese was present mainly as Mn2+ species on the glass network. A hydroxycarbonate apatite (HCA) layer was observed after 2 weeks immersion of the glasses in simulated body fluid (SBF), and a sustained Mn release was observed. MTT cell viability was assessed using human mesenchymal stromal cells (hMSCs) to evaluate the cell viability of the glass ionic products, and a cell-friendly environment was observed for all samples up to 7 days study. Osteoblastic differentiation markers were also observed and showed an increased expression of collagen type 1, osteocalcin and osteopontin after 21 days of culture for Mn-containing bioactive glasses when compared to control and 58S bioactive glass both in basal and osteogenic conditions. Results indicate that incorporating Mn in the bioactive glass is a potential strategy to obtain superior materials for tissue engineering. 
Acknowledgments: The authors gratefully acknowledge financial support from CNPq, CAPES and FAPEMIG/Brazil
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