|02-075||María Eugenia Contreras-García||Contreras-García, M.E.(Universidad Michoacana de San Nicolás de Hidalgo);||Evaluation of Cerium-doped TiO2 nanoparticles as anticancer release systems
A. Torres-Romero1, M. Cajero-Juárez2, R. E. Nuñez-Anita2 M. E. Contreras-García1
1Instituto de Investigaciones en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, C.U. Edificio “U”, C. P. 58060. Morelia, Mich, México.
2Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana San Nicolás de Hidalgo, Km
9.5, carretera Morelia-Zinapécuaro, Posta Veterinaria, Morelia, Michoacán, México, Telefax: (443) 295 8029
Since the nanoparticles were proposed for the first time as drug delivery systems –DDS–, in the 80's , their use in oncological treatment has been exploring. In the same sense, ceramic nanostructures, due their unique properties, have demonstrated potential as nanocarriers of drug molecules to target cells or tissues. In the present work, we report the use of Cerium-doped TiO2 nanoparticles –TiO2/CeO2NPs–, synthesized via sol-gel method in a microemulsion of reverse micelle, as DDS of Daunorubicin in the neoplastic cell line RPMI 8226 (ATCC® CCL-155 ™). The characterization of the nanocomposite DNR- NPsTiO2/CeO2 was carried out by UV-Vis, IR and Raman spectroscopy techniques. The experimental results revealed an efficiency load efficiency of 80.86% ± 2.14% of the drug to NPsTiO2/CeO2, much higher than that reported for NPsTiO2. In vitro cytotoxicity assays showed an improvement anticancer effect of DNR when it is associated to nanoparticles, suggesting that the nanoparticles increase intracellular deliver of drug. Likewise, it was observed that the nanoparticles proved to be biocompatible to the cells, which favors its use in medical applications, in this case as a drug carrier. TiO2/CeO2NPs showed high biocompatibility.